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Cells were then incubated with continuous shaking at 45C for 30 min, before plating on HC medium containing 2% (w/v) glucose as carbon source lacking histidine to ensure plasmid retention

Rogan later fact-checked the claims, and in a subsequent episode roasted Asprey for saying Bulletproof was toxin-free while other coffees were not, which isnt true: the coffee industry safeguards against mold toxicity, which has only been found in minuscule amounts

These are not necessarily drawbacks - they reflect the company's current business model and scope

After the mice were sacrificed, their livers were collected and weighed